Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 175, Issue 1, Pages 88-95Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2008.08.004
Keywords
Microinjection; Neuron; RNAi; Antisense oligonucleotide; Antibody; Fusion protein; Peptide
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Funding
- Deutsche Forschungsgerneinschaft [KN556/1-3, KN556/3-1, KN556/4-1]
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Functional studies in neurons often require controllable simultaneous delivery of different molecules to individual cells within networks. Microinjection represents a suitable and alternative method to deliver cDNAs, oligonucleotides,siRNAs, peptides or antibodies for expression, expression knockdown or loss-of-function studies, respectively. Moreover, molecules can be systematically applied to individual neurons in a controlled manner without affecting neighbouring cells. Establishment of microinjection is often complicated and time consuming. Here we describe a simple and reliable protocol for molecular cell biologists to establish injection of various molecules (ng to mu g range) to living neurons in a reasonable period of time. (C) 2008 Elsevier B.V. All rights reserved.
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