Journal
JOURNAL OF NEUROSCIENCE
Volume 34, Issue 21, Pages 7047-7058Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2526-13.2014
Keywords
calyx of Held; FHM-1; in vivo; P/Q calcium channels; synaptic transmission
Categories
Funding
- Wellcome Trust (UK) [084636]
- UBACYT [X-223]
- PICT BID [1728 OC.AR.PICT 2005 N 32113]
- FONCYT (ANPCyT) PICT BID (Argentina) [1728 OC.AR.PICT 2006 N 199]
- Dutch Fund for Economic Structure Reinforcement (FES) [0908]
- MRC [MR/K005170/1] Funding Source: UKRI
- Medical Research Council [MR/K005170/1] Funding Source: researchfish
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Specific missense mutations in the CACNA1A gene, which encodes a subunit of voltage-gated Ca(V)2.1 channels, are associated with familial hemiplegic migraine type 1 (FHM1), a rare monogenic subtype ofcommonmigraine with aura. Weused transgenic knock-in (KI) mice harboring the human pathogenic FHM1 mutation S218L to study presynaptic Ca2+ currents, EPSCs, and in vivo activity at the calyx of Held synapse. Whole-cell patch-clamp recordings of presynaptic terminals from S218L KI mice showed a strong shift of the calcium current I-V curve to more negative potentials, leading to an increase in basal [Ca2+](i), increased levels of spontaneous transmitter release, faster recovery from synaptic depression, and enhanced synaptic strength despite smaller action-potential-elicited Ca2+ currents. The gain-of-function of transmitter release of the S218L mutant was reproduced in vivo, including evidence for an increased release probability, demonstrating its relevance for glutamatergic transmission. This synaptic phenotype may explain the misbalance between excitation and inhibition in neuronal circuits resulting in a persistent hyperexcitability state and other migraine-relevant mechanisms such as an increased susceptibility to cortical spreading depression.
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