Journal
JOURNAL OF NEUROSCIENCE
Volume 34, Issue 19, Pages 6596-6605Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4438-13.2014
Keywords
calcium imaging; in vivo adaptive optics imaging; intrinsic signal imaging; primate fovea; retinal ganglion cells
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Funding
- National Institutes of Health [EY014375, EY01319]
- National Science Foundation Science and Technology Center for Adaptive Optics
- University of California Santa Cruz [AST-9876783]
- National Eye Institute Training [EY07125]
- Foundation Fighting Blindness and Research
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The fovea dominates primate vision, and its anatomy and perceptual abilities are well studied, but its physiology has been little explored because of limitations of current physiological methods. In this study, we adapted a novel in vivo imaging method, originally developed in mouse retina, to explore foveal physiology in the macaque, which permits the repeated imaging of the functional response of many retinal ganglion cells (RGCs) simultaneously. Agenetically encoded calcium indicator, G-CaMP5, was inserted into foveal RGCs, followed by calcium imaging of the displacement of foveal RGCs from their receptive fields, and their intensity-response functions. The spatial offset of foveal RGCs from their cone inputs makes this method especially appropriate for fovea by permitting imaging of RGC responses without excessive light adaptation of cones. This new method will permit the tracking of visual development, progression of retinal disease, or therapeutic interventions, such as insertion of visual prostheses.
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