Journal
JOURNAL OF NEUROSCIENCE
Volume 31, Issue 19, Pages 7049-7059Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.6546-10.2011
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Funding
- Deutsche Forschungsgemeinschaft
- Federal Ministry of Education and Research (BMBF) [KFO 177, TP4]
- National Institutes of Health [AG13956]
- American Health Assistance Foundation [2010613]
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Liver X receptors (LXRs) regulate immune cell function and cholesterol metabolism, both factors that are critically involved in Alzheimer's disease (AD). To investigate the therapeutic potential of long-term LXR activation in amyloid-beta (A beta) peptide deposition in an AD model, 13-month-old, amyloid plaque-bearing APP23 mice were treated with the LXR agonist TO901317. Postmortem analysis demonstrated that TO901317 efficiently crossed the blood-brain barrier. Insoluble and soluble A beta levels in the treated APP23 mice were reduced by 80% and 40%, respectively, compared with untreated animals. Amyloid precursor protein (APP) processing, however, was hardly changed by the compound, suggesting that the observed effects were instead mediated by A beta disposal. Despite the profound effect on A beta levels, spatial learning in the Morris water maze was only slightly improved by the treatment. ABCA1 (ATP-binding cassette transporter 1) and apolipoprotein E (ApoE) protein levels were increased and found to be primarily localized in astrocytes. Experiments using primary microglia demonstrated that medium derived from primary astrocytes exposed to TO901317 stimulated phagocytosis of fibrillar A beta. Conditioned medium from TO901317-treated ApoE(-/-) or LXR alpha(-/-) astrocytes did not increase phagocytosis of A beta. In APP23 mice, long-term treatment with TO901317 strongly increased the association of microglia and A beta plaques. Short-term treatment of APP/PS1 mice with TO901317 also increased this association, which was dependent on the presence of LXR alpha and was accompanied by increased ApoE lipidation. Together, these data suggest that astrocytic LXR alpha activation and subsequent release of ApoE by astrocytes is critical for the ability of microglia to remove fibrillar A beta in response to treatment with TO901317.
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