Journal
JOURNAL OF NEUROSCIENCE
Volume 31, Issue 50, Pages 18237-18250Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4554-11.2011
Keywords
-
Categories
Funding
- Spanish Ministry of Science and Innovation [BFU2008-00611, CSD2007-00023, SAF2008-03194-E]
- Gobierno Vasco fellowship
- Ramon y Cajal
- Spanish Ministry of Science and Innovation
Ask authors/readers for more resources
Long-lasting forms of neuronal plasticity require de novo gene expression, but relatively little is known about the events that occur genome-wide in response to activity in a neuronal network. Here, we unveil the gene expression programs initiated in mouse hippocampal neurons in response to different stimuli and explore the contribution of four prominent plasticity-related transcription factors (CREB, SRF, EGR1, and FOS) to these programs. Our study provides a comprehensive view of the intricate genetic networks and interactions elicited by neuronal stimulation identifying hundreds of novel downstream targets, including novel stimulus-associated miRNAs and candidate genes that may be differentially regulated at the exon/promoter level. Our analyses indicate that these four transcription factors impinge on similar biological processes through primarily non-overlapping gene-expression programs. Meta-analysis of the datasets generated in our study and comparison with publicly available transcriptomics data revealed the individual and collective contribution of these transcription factors to different activity-driven genetic programs. In addition, both gain-and loss-of-function experiments support a pivotal role for CREB in membrane-to-nucleus signal transduction in neurons. Our data provide a novel resource for researchers wanting to explore the genetic pathways associated with activity-regulated neuronal functions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available