Journal
JOURNAL OF NEUROSCIENCE
Volume 30, Issue 43, Pages 14440-14445Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3310-10.2010
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- National Institute of Neurological Disorders and Stroke [NS045260]
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Brain-derived neurotrophic factor (BDNF) facilitates the formation of long-term potentiation (LIP) in hippocampus, but whether this involves release from presynaptic versus postsynaptic pools is unclear. We therefore tested whether BDNF is essential for LIP in dorsal striatum, a structure in which the neurotrophin is present only in afferent terminals. Whole-cell recordings were collected from medium spiny neurons in striatal slices prepared from adult mice. High-frequency stimulation (HFS) of neocortical afferents produced a rapid and stable NMDA receptor-dependent potentiation. The ratio of AMPA to NMDA receptor-mediated components of the EPSPs was substantially increased after inducing potentiation, suggesting that the response enhancement involved postsynaptic changes. In accord with this, paired-pulse response ratios, a measure of transmitter release kinetics, were reduced by elevated calcium but not by LTP. Infusion of the BDNF scavenger TrkB-Fc blocked the formation of potentiation, beginning with the second minute after HFS, without reducing responses to HFS. These results suggest that presynaptic pools of BDNF can act within 2 min of HFS to support the formation of a postsynaptic form of LIP in striatum.
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