Journal
JOURNAL OF NEUROSCIENCE
Volume 30, Issue 3, Pages 1064-1072Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3909-09.2010
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Funding
- National Institutes of Health [MH07793]
- Alfred P. Sloan research fellowship
- National Alliance for Research on Schizophrenia and Depression Young Investigator Award
- Esther A. and Joseph Klingenstein Fund
- Edward Mallinckrodt Jr. Foundation
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Glutamate is a major excitatory neurotransmitter in the vertebrate brain. AMPA-type glutamate receptors mediate fast excitatory transmission. AMPA receptors assemble with transmembrane AMPA receptor regulatory protein ( TARP) auxiliary subunits and function as native ion channels. However, the assembly and stoichiometry of AMPA receptor and TARP complexes remain unclear. Here, we developed a novel strategy to determine the assembly and stoichiometry of this protein complex and found that functional AMPA receptors indeed assembled as a tetramer in a dimer-of-dimers structure. Furthermore, we found that the AMPA receptor auxiliary subunit, TARP, had a variable stoichiometry (1-4 TARP units) on AMPA receptors and that 1 TARP unit was sufficient to modulate AMPA receptor activity. In neurons, TARP had fixed and minimum stoichiometry on AMPA receptors. This fundamental composition of the AMPA receptor/TARP complex is important for the elucidation of the molecular machinery that underlies synaptic transmission.
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