Journal
JOURNAL OF NEUROSCIENCE
Volume 30, Issue 44, Pages 14635-14648Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1729-10.2010
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Funding
- Adelson Medical Research Foundation
- Mathers Charitable Foundation
- James S. McDonnell Foundation
- New York State Stem Cell Science Board
- National Institute of Neurological Disorders and Stroke [P01NS050315, R01NS33106, R01NS39559]
- AIRC (Associazione Italiana Ricerca sul Cancro
- MIUR (Minestero dell' Istruzione, dell' Universita e della Ricerca)
- FBML (Fondazione Banca del Monte di Lombardia)
- Regione Lombardia
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Sox2 is expressed by neural stem and progenitor cells, and a sox2 enhancer identifies these cells in the forebrains of both fetal and adult transgenic mouse reporters. We found that an adenovirus encoding EGFP placed under the regulatory control of a 0.4 kb sox2 core enhancer selectively identified multipotential and self-renewing neural progenitor cells in dissociates of human fetal forebrain. Upon EGFP-based fluorescence-activated cell sorting (FACS), the E/sox2:EGFP(+) isolates were propagable for up to 1 year in vitro, and remained multilineage competent throughout. E/sox2:EGFP(+) cells expressed more telomerase enzymatic activity than matched E/sox2: EGFP-depleted populations, and maintained their telomeric lengths with successive passage. Gene expression analysis of E/sox2:EGFP-sorted neural progenitor cells, normalized to the unsorted forebrain dissociates from which they derived, revealed marked overexpression of genes within the notch and wnt pathways, and identified multiple elements of each pathway that appear selective to human neural progenitors. Sox2 enhancer-based FACS thus permits the prospective identification and direct isolation of a telomerase-active population of neural stem cells from the human fetal forebrain, and the elucidation of both the transcriptome and dominant signaling pathways of these critically important cells.
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