4.7 Article

Sensory Axon-Derived Neuregulin-1 Is Required for Axoglial Signaling and Normal Sensory Function But Not for Long-Term Axon Maintenance

Journal

JOURNAL OF NEUROSCIENCE
Volume 29, Issue 24, Pages 7667-7678

Publisher

SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.6053-08.2009

Keywords

-

Categories

Funding

  1. Medical Research Council
  2. German Science Foundation [SFB 665]
  3. Acorda Therapeutics, Inc
  4. [077074/z/05/z]
  5. BBSRC [BB/F000227/1] Funding Source: UKRI
  6. MRC [G9717869] Funding Source: UKRI
  7. Biotechnology and Biological Sciences Research Council [BB/F000227/1] Funding Source: researchfish
  8. Medical Research Council [G9717869] Funding Source: researchfish

Ask authors/readers for more resources

Neuregulin-1 has a key role in mediating signaling between axons and Schwann cells during development. A limitation to studying its role in adulthood is the embryonic lethality of global Nrg1 gene deletion. We used the Cre-loxP system to generate transgenic mice in which neuregulin-1 is conditionally ablated in the majority of small-diameter and a proportion of large-diameter sensory neurons that have axons conducting in the C- and A delta-fiber range, respectively. Sensory neuron-specific neuregulin-1 ablation resulted in abnormally large Remak bundles with axons clustered in polyaxonal pockets. The total number of axons in the sural nerve was unchanged, but a greater proportion was unmyelinated. In addition, we observed large-diameter axons that were in a 1: 1 relationship with Schwann cells, surrounded by a basal lamina but not myelinated. There was no evidence of DRG or Schwann cell death; the markers of different DRG cell populations and cutaneous innervation were unchanged. These anatomical changes were reflected in a slowing of conduction velocity at the lower end of the A-fiber conduction velocity range and a new population of more rapidly conducting C-fibers that are likely to represent large-diameter axons that have failed to myelinate. Conditional neuregulin-1 ablation resulted in a reduced sensitivity to noxious mechanical stimuli. These findings emphasize the importance of neuregulin-1 in mediating the signaling between axons and both myelinating and nonmyelinating Schwann cells required for normal sensory function. Sensory neuronal survival and axonal maintenance, however, are not dependent on axon-derived neuregulin-1 signaling in adulthood.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available