Journal
JOURNAL OF NEUROSCIENCE
Volume 28, Issue 36, Pages 8908-8913Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1526-08.2008
Keywords
dopamine; ventral tegmental area; nucleus accumbens; amygdala; D-2 receptor; kappa-opioid
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Funding
- State of California for medical research on alcohol and substance abuse
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Broad action potentials (APs) and dopamine (DA) D2 receptor (D2R)-mediated inhibition are widely used to identify midbrain DA neurons. However, when these measures are taken alone they do not predict DA content in ventral tegmental area (VTA) neurons. In fact, some VTA neuronal properties correlate better with projection target than neurotransmitter content. Here we report that amygdala (AMYG)-projecting VTA DA neurons have brief APs and lack D2R agonist (quinpirole; 1 mu M) autoinhibition. However, they are hyperpolarized by both the GABA(B) agonist baclofen ( 1 mu M) and the kappa-opioid receptor agonist U69593 [(+)-(5 alpha,7 alpha,8 beta)-N-methyl-N-[7-(1-pyrrolidinyl)-1-oxaspiro[4.5]dec-8-yl]benzeneacetamide; 1 mu M]. Furthermore, we show that accurate prediction of DA content in VTA neurons is possible when the projection target is known: in both nucleus accumbens- and AMYG-projecting neural populations, AP durations are significantly longer in DA than non-DA neurons. Among prefrontal cortex-projecting neurons, quinpirole sensitivity, but not AP duration, is a predictor of DA content. Therefore, in the VTA, AP duration and inhibition by D2R agonists may be valid markers of DA content in neurons of known projection target.
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