Journal
JOURNAL OF NEUROSCIENCE
Volume 28, Issue 50, Pages 13574-13581Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4099-08.2008
Keywords
Nrf2; astrocytes; glutathione; motor neurons; neuronal death; neuroprotection
Categories
Funding
- ALS Association
- Robert Packard Center for ALS Research at Johns Hopkins
- National Institute of Environmental Health Sciences [ES08089, ES10042, NS060120, HD03352]
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Activation of the transcription factor Nrf2 in astrocytes coordinates the upregulation of antioxidant defenses and confers protection to neighboring neurons. Dominant mutations in Cu/Zn-superoxide dismutase (SOD1) cause familial forms of amyotrophic lateral sclerosis (ALS), a fatal disorder characterized by the progressive loss of motor neurons. Non-neuronal cells, including astrocytes, shape motor neuron survival in ALS and are a potential target to prevent motor neuron degeneration. The protective effect of Nrf2 activation in astrocytes has never been examined in a chronic model of neurodegeneration. We generated transgenic mice over-expressing Nrf2 selectively in astrocytes using the glial fibrillary acidic protein (GFAP) promoter. The toxicity of astrocytes expressing ALS-linked mutant hSOD1 to cocultured motor neurons was reversed by Nrf2 over-expression. Motor neuron protection depended on increased glutathione secretion from astrocytes. This protective effect was also observed by crossing the GFAP-Nrf2 mice with two ALS-mouse models. Overexpression of Nrf2 in astrocytes significantly delayed onset and extended survival. These findings demonstrate that Nrf2 activation in astrocytes is a viable therapeutic target to prevent chronic neurodegeneration.
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