Journal
JOURNAL OF NEUROSCIENCE
Volume 28, Issue 19, Pages 5072-5081Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4476-07.2008
Keywords
cancer-induced pain; heat hyperalgesia; primary afferent neurons; TNF alpha; TNF receptor type 2; TRPV1
Categories
Ask authors/readers for more resources
To provide a tool to investigate the mechanisms inducing and maintaining cancer-related pain and hyperalgesia, a soft tissue tumor/metastasis model was developed that is applicable in C57BL/6J wild-type and transgenic mice. We show that the experimental tumor-induced heat hyperalgesia and nociceptor sensitization were prevented by systemic treatment with the tumor necrosis factor alpha (TNF alpha) antagonist etanercept. In naive mice, exogenous TNF alpha evoked heat hyperalgesia in vivo and sensitized nociceptive nerve fibers to heat in vitro. TNF alpha enhanced the expression of the nociceptor-specific heat transducer ion channel transient receptor potential vanilloid 1 (TRPV1) and increased the amplitudes of capsaicin and heat-activated ionic currents via p38/MAP (mitogen-activated protein) kinase and PKC (protein kinase C). Deletion of the tumor necrosis factor receptor type 2 (TNFR2) gene attenuated heat hyperalgesia and prevented TRPV1 upregulation in tumor-bearing mice, whereas TNFR1 gene deletion played a minor role. We propose endogenousTNF alpha as a key player in cancer-related heat hyperalgesia and nociceptor sensitization that generates TRPV1 upregulation and sensitization via TNFR2.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available