Journal
JOURNAL OF NEUROSCIENCE
Volume 28, Issue 29, Pages 7344-7349Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1043-08.2008
Keywords
addiction; amphetamine; striatum; chromatin; histone modification; gene regulation
Categories
Funding
- NIDA NIH HHS [R01 DA007359] Funding Source: Medline
- NIMH NIH HHS [R01 MH051399-16, R01 MH051399] Funding Source: Medline
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The molecular mechanisms underlying the transition from recreational drug use to chronic addiction remain poorly understood. One molecule implicated in this process is Delta FosB, a transcription factor that accumulates in striatum after repeated drug exposure and mediates sensitized behavioral responses to psychostimulants and other drugs of abuse. The downstream transcriptional mechanisms by which Delta FosB regulates drug-induced behaviors are incompletely understood. We reported previously the chromatin remodeling mechanisms by which Delta FosB activates the expression of certain genes; however, the mechanisms underlying Delta FosB-mediated gene repression remain unknown. Here, we identify c-fos, an immediate early gene rapidly induced in striatum after acute psychostimulant exposure, as a novel downstream target that is repressed chronically by Delta FosB. We show that accumulation of Delta FosB in striatum after chronic amphetamine treatment desensitizes c-fos mRNA induction to a subsequent drug dose. Delta FosB desensitizes c-fos expression by recruiting histone deacetylase 1 (HDAC1) to the c-fos gene promoter, which, in turn, deacetylates surrounding histones and attenuates gene activity. Accordingly, local knock-out of HDAC1 in striatum abolishes amphetamine-induced desensitization of the c-fos gene. In concert, chronic amphetamine increases histone H3 methylation on the c-fos promoter, a chromatin modification also known to repress gene activity, as well as expression levels of the H3 histone methyltransferase, KMT1A (lysine methyltransferase 1A, formerly SUV39H1). This study reveals a novel epigenetic pathway through which Delta FosB mediates distinct transcriptional programs that may ultimately alter behavioral plasticity to chronic amphetamine exposure.
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