Journal
JOURNAL OF NEUROPHYSIOLOGY
Volume 111, Issue 5, Pages 1132-1149Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00785.2013
Keywords
local field potential; unit firing; monosynaptic connections; behaving rats and mice
Categories
Funding
- National Institutes of Health [NS-34994, MH-54671, NS-074015]
- Human Frontier Science Program
- J. D. McDonnell Foundation
- Marie Curie FP7-PEOPLE-IOF Grant [254780]
- EU-FP7-ERC-Starting Grant [337075]
- Momentum Program of the Hungarian Academy of Sciences
- Rosztoczy Foundation
- European Research Council (ERC) [337075] Funding Source: European Research Council (ERC)
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Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized micro-drive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.
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