Neural dynamics in response to binary taste mixtures

Maier JX, Katz DB. Neural dynamics in response to binary taste mixtures. J Neurophysiol 109: 2108-2117, 2013. First published January 30, 2013; doi:10.1152/jn.00917.2012.-Taste stimuli encountered in the natural environment are usually combinations of multiple tastants. Although a great deal is known about how neurons in the taste system respond to single taste stimuli in isolation, less is known about how the brain deals with such mixture stimuli. Here, we probe the responses of single neurons in primary gustatory cortex (GC) of awake rats to an array of taste stimuli including 100% citric acid (100 mM), 100% sodium chloride (100 mM), 100% sucrose (100 mM), and a range of binary mixtures (90/10, 70/30, 50/50, 30/70, and 10/90%). We tested for the presence of three different hypothetical response patterns: 1) responses varying monotonically as a function of concentration of sucrose (or acid) in the mixture (the monotonic pattern); 2) responses increasing or decreasing as a function of degree of mixture of the stimulus (the mixture pattern); and 3) responses that change abruptly from being similar to one pure taste to being similar the other (the categorical pattern). Our results demonstrate the presence of both monotonic and mixture patterns within responses of GC neurons. Specifically, further analysis (that included the presentation of 50 mM sucrose and citric acid) made it clear that mixture suppression reliably precedes a palatability-related pattern. The temporal dynamics of the emergence of the palatability-related pattern parallel the temporal dynamics of the emergence of preference behavior for the same mixtures as measured by a brief access test. We saw no evidence of categorical coding.