Journal
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
Volume 73, Issue 8, Pages 798-806Publisher
OXFORD UNIV PRESS INC
DOI: 10.1097/NEN.0000000000000099
Keywords
Astrocyte; Cortical dysplasia; Epilepsy; Metabotropic receptor; Potassium channels; Purinergic receptor
Categories
Funding
- Intramural Research Grants for Neurological and Psychiatric Disorders of National Center of Neurology and Psychiatry [22A3, 25-3]
- Ministry of Health, Labour and Welfare of Japan (Research on Rare and Intractable Diseases) [24-078]
- Comprehensive Research on Disability Health and Welfare (Neurological and Muscle Diseases) [24-007]
- Japan Society for the Promotion of Science [25670486, 24390270]
- Grants-in-Aid for Scientific Research [25117003, 24390270, 25670486, 25670622] Funding Source: KAKEN
Ask authors/readers for more resources
Epilepsy is one of the major neurologic diseases, and astrocytes play important roles in epileptogenesis. To investigate possible roles of astrocyte-related receptors in patients with intractable epilepsy associated with focal cortical dysplasia (FCD) and other conditions, we examined resected epileptic foci from 31 patients, including 23 with FCD type I, IIa, or IIb, 5 with tuberous sclerosis complex, and 3 with low-grade astrocytoma. Control samples were from 21 autopsied brains of patients without epilepsy or neurologic deficits and 5 patients with pathologic gliosis without epilepsy. Immunohistochemical and immunoblot analyses with antibodies against purinergic receptor subtypes P2RY1, P2RY2, P2RY4, potassium channels Kv4.2 and Kir4.1, and metabotropic receptor subtypes mGluR1 and mGluR5 were performed. Anti-glial fibrillary acidic protein, anti-NeuN, and anti-CD68 immunostaining was used to identify astrocytes, neurons, and microglia, respectively. Most glial fibrillary acidic protein-immunopositive astrocyte cells in the brain samples from patients with epilepsy were P2RY1-, P2RY2-, P2RY4-, Kv4.2-, Kir4.1-, mGluR1-, and mGluR5-positive, whereas samples from controls and pathologic gliosis showed lower expression levels of these astrocyte-related receptors. Our findings suggest that, although these receptors are necessary for astrocyte transmission, formation of the neuron-glia network, and other physiologic functions, overexpression in the brains of patients with intractable epilepsy may be associated with activation of intracellular and glio-neuronal signaling pathways that contribute to epileptogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available