Journal
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
Volume 72, Issue 7, Pages 624-645Publisher
OXFORD UNIV PRESS INC
DOI: 10.1097/NEN.0b013e31829768d2
Keywords
CD4 cells; Foxp3; GDNF; Major histocompatibility complex molecules; Microglia; Parkinson disease; Vaccination
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Funding
- Lundbeck Foundation
- Danish Research Council
- Danish Parkinson Foundation
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Human leukocyte antigen-DR induction and lymphocyte infiltrates in the brains of patients with Parkinson disease (PD) and the presence in serum of alpha-synuclein (alpha-syn)-specific antibodies suggest that the peripheral immune system may have an active role in the progression of PD. We designed a vaccination strategy to attempt to control these processes and mediate protection against disease progression in a rat PD model. Using a recombinant adeno-associated viral vector, we unilaterally overexpressed human alpha-syn in the rat substantia nigra to induce a progressive neuropathologic process. Prior to stereotactic delivery of the viral vector, animals were vaccinated with recombinant alpha-syn (asyn). This resulted in a high-titer anti-alpha-syn antibody response on alpha-syn overexpression; the accumulation of CD4-positive, MHC II-positive ramified microglia in the substantia nigra; long-lasting infiltration of CD4-positive, Foxp3-positive cells throughout the nigrostriatal system; and fewer pathologic aggregates in the striatum versus control animals that had received a mock vaccine. A longterm increase in GDNF levels in the striatum and IgG deposition in alpha-syn-overexpressing cells and neurites in the substantia nigra were also observed. Together, these results suggest that a protective vaccination strategy results in induction of regulatory T cells and distinctly activated microglia, and that this can induce immune tolerance against alpha-syn.
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