Journal
JOURNAL OF NEUROINFLAMMATION
Volume 11, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/s12974-014-0143-1
Keywords
Dendritic cells; Injury; Microglia; Retinal ganglion cells; NF kappa B
Categories
Funding
- Wallin Neuroscience Discovery Fund (DSG)
- Minnesota Lions Clubs
- Research to Prevent Blindness, Inc.
- [R01-EY021003]
- [T32-EY07133]
- [T32-AG029796]
- [P30-EY011374]
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Background: Immune system cells are known to affect loss of neurons due to injury or disease. Recruitment of immune cells following retinal/CNS injury has been shown to affect the health and survival of neurons in several models. We detected close, physical contact between dendritic cells and retinal ganglion cells following an optic nerve crush, and sought to understand the underlying mechanisms. Methods: CD11c-DTR/GFP mice producing a chimeric protein of diphtheria toxin receptor (DTR) and GFP from a transgenic CD11c promoter were used in conjunction with mice deficient in MyD88 and/or TRIF. Retinal ganglion cell injury was induced by an optic nerve crush, and the resulting interactions of the GFP(hi) cells and retinal ganglion cells were examined. Results: Recruitment of GFP(hi) dendritic cells to the retina was significantly compromised in MyD88 and TRIF knockout mice. GFP(hi) dendritic cells played a significant role in clearing fluorescent-labeled retinal ganglion cells post-injury in the CD11c-DTR/GFP mice. In the TRIF and MyD88 deficient mice, the resting level of GFP(hi) dendritic cells was lower, and their influx was reduced following the optic nerve crush injury. The reduction in GFP(hi) dendritic cell numbers led to their replacement in the uptake of fluorescent-labeled debris by GFP(lo) microglia/macrophages. Depletion of GFP(hi) dendritic cells by treatment with diphtheria toxin also led to their displacement by GFP(lo) microglia/macrophages, which then assumed close contact with the injured neurons. Conclusions: The contribution of recruited cells to the injury response was substantial, and regulated by MyD88 and TRIF. However, the presence of these adaptor proteins was not required for interaction with neurons, or the phagocytosis of debris. The data suggested a two-niche model in which resident microglia were maintained at a constant level post-optic nerve crush, while the injury-stimulated recruitment of dendritic cells and macrophages led to their transient appearance in numbers equivalent to or greater than the resident microglia.
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