Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 225, Issue 1-2, Pages 91-99Publisher
ELSEVIER
DOI: 10.1016/j.jneuroim.2010.04.023
Keywords
Interferon-gamma receptor; Microglia; Astrocytes; Oligodendrocytes; Neurodegenerative diseases
Categories
Funding
- Pacific Alzheimer Research Foundation
- Jack Brown and Family Alzheimer's Disease Research Foundation
- Natural Sciences and Engineering Research Council of Canada
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Although significant effects of interferon-gamma (IFN-gamma) on glial cells are well documented, information on the expression level and localization of glial IFN-gamma receptors (IFN-gamma-R) in the human central nervous system (CNS) is sparse. To examine the glial expression of IFN-gamma-R in the human CNS, immunohistochemistry and quantitative analyses were performed on Alzheimer disease hippocampus. Parkinson disease substantia nigra, amyotrophic lateral sclerosis spinal cord and corresponding areas from non-neurological cases. Almost all IFN-gamma-R-positive (IFN-gamma-R+) cells corresponded to GFAP-positive (GFAP(+)) astrocytes, while none of IFN-gamma-R+ cells corresponded to IBA1-positive (IBA1(+)) microglia or MBP-positive (MBP+) oligodendrocytes in these neurological cases. We observed a similar pattern of glial IFN-gamma-R expression in non-neurological cases. Also, we quantitatively analyzed the IFN-gamma-R expression by cultured human glial cells using immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). In contrast to in vivo results, almost all IFN-gamma-R+ cells were IBA1(+) in microglial cultures. GFAP(+) in astrocytic cultures and MBP+ in oligodendrocytic cultures. Moreover, no significant difference in IFN-gamma-R mRNA expression was found for these glial cell types by RT-PCR. These results suggest that the microglial and oligodendrocytic expression levels of IFN-gamma-R are much lower than the astrocytic expression levels in the human CNS in vivo, whereas all three types of glial cells constitutively express IFN-gamma-R when cultured in vitro. (C) 2010 Elsevier B.V. All rights reserved.
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