Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 211, Issue 1-2, Pages 110-113Publisher
ELSEVIER
DOI: 10.1016/j.jneuroim.2009.04.001
Keywords
Anti-aquaporin-4 antibodies; NMO; Chimera mutant AQP4; Epitope; Extracellular domain
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Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Ministry of Health, Labour and Welfare, Japan
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Objective: Anti-aquaporin 4 antibodies (AQP4-Ab) are specifically detected in patients with neuromyelitis optica. To investigate the role of AQP4-Ab, we examined the antibody binding epitope using human and mouse mutant AQP4. Methods: We constructed human and mouse amino acid substitution AQP4 mutants and compared the reactivity with wild-form of human, mouse and rat AQP4. Results: The decreased intensity of AQP4-Ab staining with mouse AQP4 was recovered to that of human AQP4 with the mouse mutant A228E for 9 of the 10 sera. Conclusions: The third extracellular loop of AQP4 is considered to be the major epitope for AQP4-Ab in NMO. (C) 2009 Elsevier B.V. All rights reserved.
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