Journal
JOURNAL OF NEUROGENETICS
Volume 22, Issue 3, Pages 285-307Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/01677060802298517
Keywords
bioluminescence; Ca2+-brain imaging; cameleon; G-CaMP; GFP-aequorin; fluorescence
Categories
Funding
- Agence National pour la Recherche
- CNRS
Ask authors/readers for more resources
In the last decade, several different optical-imaging techniques have been developed to study neuronal activity, with the aim to map and decipher the neural code underlying major neurophysiologic functions, such as odor perception, learning and memory, locomotor activity, and sleep, to name a few. The first generation of these techniques was principally based on detecting either transmembrane voltage or calcium activity by using fluorescent dye markers. Recently, the development of genetically encoded probes has extended the limits, increased the accessibility of deeper structures, and more importantly, allowed investigators to precisely label and identify the desired neurons. However, several deep structures of the brain still remain refractive to these approaches, suggesting that the development of other techniques will be welcome. Recently, a new bioluminescence approach has been described, and although it is still a new technique, the first reported results, and the biological phenomena that have been revealed, make it extremely promising. This review will summarize the recent progress of these different imaging approaches, comparing the limits and constraints of each of them, and will guide the reader in choosing the most appropriate method in accordance with the desired neurons or functions under investigation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available