Journal
JOURNAL OF NEUROCHEMISTRY
Volume 119, Issue 4, Pages 686-696Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1471-4159.2011.07465.x
Keywords
astrocyte; calcium; GDNF; ifenprodil; IL-1 beta; NMDA
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Funding
- Swedish Research Council [33X-06812]
- Edit Jacobson's Foundation
- Folksam's Forskningsstiftelse
- Arvid Carlsson's Foundation
- Sahlgrenska University Hospital, Gothenburg, Sweden [LUA/ALF GBG-11587]
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Glial cell line-derived neurotrophic factor (GDNF) plays an important role in neuroinflammatory and neuropathic pain conditions. Astrocytes produce and secrete GDNF, which interacts with its receptors to induce Ca2+ transients. This study aimed first to assess intracellular Ca2+ responses of astrocytes in primary culture when exposed to the neuroprotective and anti-inflammatory peptide GDNF. Furthermore, incubation with the inflammatory inducers lipopolysaccharide (LPS), NMDA, or interleukin 1-beta (IL-1 beta) attenuated the GDNF-induced Ca2+ transients. The next aim was to try to restore the suppressed GDNF responses induced by inflammatory changes in the astrocytes with an anti-inflammatory substance. Ifenprodil, an NMDA receptor antagonist at the NR2B subunit, was tested. It was shown to restore the GDNF-evoked Ca2+ transients and increased the Na+/K+-ATPase expression. Ifenprodil seems to be a potent anti-inflammatory substance for astrocytes which have been pre-activated by inflammatory stimuli.
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