Journal
JOURNAL OF NEUROCHEMISTRY
Volume 115, Issue 1, Pages 283-295Publisher
WILEY
DOI: 10.1111/j.1471-4159.2010.06928.x
Keywords
ADORA2A; DNA methylation; YY1; ZBP-89
Categories
Funding
- Ministerio de Ciencia e Innovacion, Instituto de Salud Carlos III [PI05/1631, CP08/00095]
- European Union [MRTN-CT-2006-035810]
- Consejeria de Educacion y Ciencia [PCI08-0125]
- Consejeria de Sanidad of Junta de Comunidades de Castilla-La Mancha [PI-2007/50, G-2007-C/13]
- Ministerio de Ciencia e Innovacion [BFU2008-00138]
- Fundacio La Marato de TV3 [092330]
- University of Barcelona
Ask authors/readers for more resources
P>Adenosine A(2A) receptors (A(2A)Rs) are G-protein coupled receptors that stimulate adenylyl cyclase activity. The most A(2A)Rs-enriched brain region is the striatum, in which A(2A)Rs are largely restricted to GABAergic neurons of the indirect pathway. We recently described how DNA methylation controls basal A(2A)R expression levels in human cell lines. The present report provides clues about the molecular mechanisms that promote human brain region-specific A(2A)R gene (ADORA2A) basal expression. The transcription factors ZBP-89 and Yin Yang-1 (YY1) have been characterized as regulators of ADORA2A in SH-SY5Y cells by means of specific expression vectors/siRNAs transient transfection and chromatin immunoprecipitation assay. ZBP-89 plays a role as an activator and YY1 as a repressor. No differences were found in ZBP-89 levels with western blot between the putamen and cerebellum of human postmortem brains. However, increased YY1 levels and DNA methylation percentage in the 5' untranslated region of ADORA2A, using SEQUENOM MassArray, were found in the cerebellum with respect to the putamen of human brains, showing an inverse relationship with A(2A)R levels in the two cerebral regions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available