Journal
JOURNAL OF NEUROCHEMISTRY
Volume 105, Issue 5, Pages 1761-1769Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1471-4159.2008.05266.x
Keywords
alpha-conotoxin; knockout mice; nicotine; striatum; voltammetry
Categories
Funding
- NIDA NIH HHS [P30 DA015663, DA015663] Funding Source: Medline
- NIGMS NIH HHS [P01 GM048677, P01 GM048677-160012, GM 48677, P01 GM048677-15] Funding Source: Medline
- NIMH NIH HHS [R01 MH053631, R29 MH053631, MH 53631, R01 MH053631-13, R01 MH053631-11S1] Funding Source: Medline
Ask authors/readers for more resources
Striatal dopamine (DA) plays a major role in the regulation of motor coordination and in the processing of salient information. We used voltammetry to monitor DA-release evoked by electrical stimulation in striatal slices, where interneurons continuously release acetylcholine. Use of the alpha 6-selective antagonist alpha-conotoxin MII[E11A] and alpha 4 knockout mice enabled identification of two populations of DA-ergic fibers. The first population had a low action potential threshold, and action potential-evoked DA-release from these fibers was modulated by alpha 6. The second population had a higher action potential threshold, and only alpha 4(non-alpha 6) modulated action potential-evoked DA-release. Striatal DA-ergic neurons fire in both tonic and phasic patterns. When stimuli were applied in a train to mimic phasic firing, more DA-release was observed in alpha 4 knockout versus wild-type mice. Furthermore, block of alpha 4(non-alpha 6), but not of alpha 6, increased DA release evoked by a train. These results indicate that there are different classes of striatal DA-ergic fibers that express different subtypes of nicotinic receptors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available