Journal
JOURNAL OF NATURAL PRODUCTS
Volume 75, Issue 4, Pages 802-806Publisher
AMER CHEMICAL SOC
DOI: 10.1021/np300034c
Keywords
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Funding
- University of Wisconsin Madison School of Pharmacy
- Graduate School at the University of Wisconsin
- U.W. College of Agriculture and Life Sciences
- NIH [GM092009]
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A lack of good methods for absolute quantification of natural products has limited the accuracy of high-throughput screening Many currently used methods for quantification are either too slow or not amenable to the structural diversity of natural products. Recent developments in low-temperature evaporative light scattering detectors (ELSD-LT) have overcome several historical limitations of ELSDs, including analyte decomposition and low sensitivity. Primarily, ELSDs have been used for relative quantification and detection of compounds that lack a UV chromophore In this study, we employ an ELSD-LT for absolute quantification of natural products, Calibration curves were constructed using a weighted least-squares analysis for a diverse set of natural products and other compounds. An average calibration curve was evaluated for the universal quantification of natural products. Optimization of ELSD-LT hardware and parameters improved sensitivity and throughput and established the utility of ELSD-LT for quantification of large natural product libraries.
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