Bis-spirolabdane Diterpenoids from Leonotis nepetaefolia

Ten new bis-spirolabdane diterpenoids, leonepetaefolins A-E (1, 3, 5, 7, 9) and 15-epi-leonepetaefolins A-E (2, 4, 6, 8, 10), together with eight known labdane diterpenoids (11-18) as well as two known flavonoids, apigenin and cirsiliol, were isolated from the leaves of Leonotis nepetaefolia. The structures of the new compounds were determined on the basis of ID- and 2D-NMR experiments including H-1, C-13, DEPT, H-1-H-1 COSY, HSQC, HMBC, and NOESY. The absolute configuration of an epimeric mixture of 1 and 2 was determined by X-ray crystallographic analysis. The compounds - isolated were evaluated for their binding propensity in several CNS G-protein-coupled receptor assays in vitro.