Hippolides A-H, Acyclic Manoalide Derivatives from the Marine Sponge Hippospongia lachne

Eight new acyclic manoalide-related sesterterpenes, hippolides A H (1-8), together with two known manoalide derivatives, (6E)-neomanoalide (9) and (6Z)-neomanoalide (10), were isolated from the South China Sea sponge Hippospongia lachne. The absolute configurations of 1-8 were established by the modified Mosher's method and CD data. Compound 1 exhibited cytotoxicity against A549, HeLa, and HCT-116 cell lines with IC50 values of 5.22 x 10(-2), 4.80 x 10(-2), and 9.78 mu M, respectively. Compound 1 also showed moderate PTP1B inhibitory activitiy with an IC50 value of 23.81 mu M, and compound 2 showed moderate cytotoxicity against the HCT-116 cell line and PTP1B inhibitory activity with IC50 values of 35.13 and 39.67 yM, respectively. In addition, compounds 1 and 5 showed weak anti-inflammatory activity, with IC50 values of 61.97 and 40.35 mu M for PKC gamma and PKC alpha, respectively.