Vasorelaxant effects on rat aortic artery by two types of indole alkaloids, naucline and cadamine

Two indole alkaloids, naucline from Nauclea officinalis and cadamine from Ochreinauclea maingayii, were individually evaluated for vasorelaxant effects on phenylephrine-precontracted aortic rings. Naucline induced concentration-dependent relaxation in aortic rings. Respective EC50 values of naucline on aorta rings with/without endothelium did not show a significant difference, indicating that naucline-induced relaxation was independent of the endothelium. In further experiments with various inhibitors, naucline was found to possess inhibitory effects on both voltage-dependent Ca2+ channel (VDC)- and receptor-operated Ca2+ channel (ROC)-dependent Ca2+ influx in smooth muscle. Cadamine showed concentration-dependent relaxation in endothelium-intact aortic rings, which was inhibited by addition of l-NMMA, NOS inhibitor. These results suggested that the vasorelaxant effect of cadamine is partly mediated through the increased release of NO from endothelial cells. In addition to NO involvement, vasorelaxation induced by cadamine was also attributed to inhibition of both VDC- and ROC-dependent Ca2+ influx.