Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 10, Issue 10, Pages 6313-6317Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2010.2625
Keywords
Silver Nanoparticle; Blood-Brain Barrier; Ultrastructure; Biocompatibility
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Funding
- National Natural Science Foundation of China
- National Institute for the Control of Pharmaceutical and Biological Products
- Institute of Neurosurgery of Chinese Academy of Medical Sciences
- National Institute for Radiological Protection and Nuclear Safety of Chinese Center for Disease Control
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Silver nanoparticles (SNPs) translocate to the brain through the blood stream after they are implanted in vivo. The aim of this study was to investigate the distribution of SNPs that crossed through the blood-brain barrier (BBB). An in vitro BBB model established by co-cultures of rat brain microvessel vascular endothelial cells (BMVECs) with astrocytes (ACs) was cultured with cell culture medium containing 100 mu g/mL of either SNPs or silver microparticles (SMPs). After 4 hours of culture, the ultrastructure and its silver content of BBB was evaluated with transmission electronic microscopy (TEM) and inductively-coupled plasma mass spectrometry (ICP-MS) respectively. Results demonstrated that SNPs crossed the BBB and accumulated inside BMVECs, while the SMPs did not. The data indicated a special distribution of SNPs in the BBB and suggested that SNPs pass the BBB mainly by transcytosis of capillary endothelial cells. Further study would be necessary to evaluate the actual biological effects of SNPs on the brain.
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