Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 10, Issue 10, Pages 6864-6868Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2010.2989
Keywords
Submicron-Pattern; Fibronectin; Human Dermal Fibroblast; Self-Assembled Monolayer; Soft Lithography
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Funding
- Korea Evaluation Institute of Industrial Technology (KEIT) [10030064] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2007-2000333] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Cell adhesion is an important step in cell survival, and in the proliferation of anchorage-dependent cells, whose dimensions can be controlled by micro-patterning of the cell-adhesive extracellular matrix. To fabricate a micro-patterned fibronectin substrate with spacings ranging from 0.9 mu m to 20 mu m, we made a replica mold using e-beam lithography. The physiological behavior of human dermal fibroblasts (HDFs) on a substrate with a gradient of pattern spacings from 0.9 mu m to 20 mu m was evaluated after 4.5 hours and 2 days of culture. The number of proliferating cells on the fibronectin-patterned surface increased as the spacing between strip lines increased to 11 mu m. However, the number of cells gradually decreased when the pattern spacing exceeded 11 mu m. These findings demonstrate that the submicron-patterned topography of a substrate plays important roles in HDF survival and proliferation.
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