Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 10, Issue 2, Pages 719-725Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2010.1906
Keywords
Nanoparticles; Sensor; Antibody; Protein A
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Funding
- Lincoln University of Missouri
- National Institute of Health/National Cancer Institute [5R01 CA119412-04]
- Nanoscience and Nanotechnology program [1R21CA128460-02]
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A one-step method for synthesis of bioconjugated gold nanoparticles is reported. A non-toxic and biocompatible phosphorus based reducing agent was used for reduction of gold (III) and formation of nanoparticles. Physicochemical properties of protein-A stabilized gold nanoparticls were investigated. Result of immunoassay experiments confirmed the potential of the synthesized anti-protein-A conjugated gold nanoparticles for use as a simple and inexpensive test for quantitative screening of protein-A samples.
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