Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 8, Issue 5, Pages 2316-2322Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2008.177
Keywords
cancer; apoferritin; yttrium-90; radioimmunotherapy; nanoparticles
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Funding
- NINDS NIH HHS [U01 NS058161-01] Funding Source: Medline
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We report a templated-synthetic approach based on protein-cage of apoferritin to prepare radionuclide nanoparticle (NP) conjugates. Non-radioactive yttrium (Y-89) was used as a model target and surrogate for radioyttrium (Y-90) to prepare the nanoparticle conjugate. The center cavity and multiple channel structure of apoferritin offer a fast and facile method to precipitate yttrium phosphate by diffusing yttrium and phosphate ions into the cavity of apoferritin, resulting a core-shell nanoparticle. The yttrium phosphate/apoferritin nanoparticle was functionalized with biotin for further application. The synthesized nanoparticle was characterized by transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). We found that the resulting nanoparticles were uniform in size, with a diameter of around 8 nm. We tested the pre-targeting capability of the biotin-modified yttrium phosphate/apoferritin nanoparticle conjugate with streptavidin-modified magnetic beads and with aid of streptavidin-modified fluorescein isothiocyanate (FITC) tracer. This work shows that an yttrium phosphate NP conjugate provides a fast, simple and efficient method to prepare radioactive yttrium conjugate for potential applications in radioimmunotherapy of cancer.
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