Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 16, Issue 4, Pages -Publisher
SPRINGER
DOI: 10.1007/s11051-014-2337-y
Keywords
Copper oxide nanoparticles; Sonication; Sebocytes; Keratinocytes; Toxicity; Inflammatory response
Categories
Funding
- DGO6 (Direction Generale Operationnelle de l'Economie, de l'Emploi et de la Recherche) of the Walloon Region of Belgium ('Nanotoxico' Pole of Excellence) [516252]
- European Commission [262163]
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The potential toxic and pro-inflammatory effects of rod-shaped copper oxide (CuO) nanoparticles (NPs; 10 +/- 3 nm in thickness and 74 +/- 17 nm in length) were studied on N-hTERT keratinocytes and SZ95 sebocytes and on reconstructed human epidermis. Non-sonicated and sonicated CuO NPs induced similar cellular toxicity. The toxic effect of CuO NPs (non-sonicated and sonicated) was more pronounced in keratinocytes than in sebocytes. Pro-oxidant effects of CuO NPs were demonstrated by showing increase in the production of reactive oxygen species and decrease of cellular glutathione. In addition, DNA-binding activities suggested that redox-sensitive transcription factors Nrf2 and NF-kappa B were implicated in the response of keratinocytes to CuO NPs. Transcriptomic analysis showed an increase in the abundance of transcript species coding for pro-inflammatory interleukins (e.g. IL-8 and IL-1 alpha) and chemokines. In reconstituted human epidermis exposed topically to raw CuO NPs, no effect on the integrity, viability and inflammatory response was noticed.
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