Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 15, Issue 8, Pages -Publisher
SPRINGER
DOI: 10.1007/s11051-013-1874-0
Keywords
Iron oxide; Dextran; Surface charge; Uptake; Endocytosis
Categories
Funding
- US NSF [CBET-0609117, OIA-0701525]
- tNIH [1 R15 EB010228-01]
- NSF-MRSEC program [DMR 0520404]
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R15EB010228] Funding Source: NIH RePORTER
- Division Of Human Resource Development [0833112] Funding Source: National Science Foundation
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Nanoparticle physicochemical properties such as surface charge are considered to play an important role in cellular uptake and particle-cell interactions. In order to systematically evaluate the role of surface charge on the uptake of iron oxide nanoparticles, we prepared carboxymethyl-substituted dextrans with different degrees of substitution, ranging from 38 to 5 groups per chain, and reacted them using carbodiimide chemistry with amine-silanecoated iron oxide nanoparticles with narrow size distributions in the range of 33-45 nm. Surface charge of carboxymethyl-substituted dextran-coated nanoparticles ranged from -50 to 5 mV as determined by zeta potential measurements, and was dependent on the number of carboxymethyl groups incorporated in the dextran chains. Nanoparticles were incubated with CaCo-2 human colon cancer cells. Nanoparticle-cell interactions were observed by confocal laser scanning microscopy and uptake was quantified by elemental analysis using inductively coupled plasma mass spectroscopy. Mechanisms of internalization were inferred using pharmacological inhibitors for fluid-phase, clathrin-mediated, and caveola-mediated endocytosis. Results showed increased uptake for nanoparticles with greater negative charge. Internalization patterns suggest that uptake of the most negatively charged particles occurs via non-specific interactions.
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