Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 14, Issue 4, Pages -Publisher
SPRINGER
DOI: 10.1007/s11051-012-0829-1
Keywords
Enzyme encapsulation; Silicate nanoparticle; HRP; IAA; Prodrug cancer therapy; Nanomedicine
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Funding
- Ministry of Education of the R.O.C. (Taiwan)
- National Science Council of the R.O.C. (Taiwan) [NSC 97-2311-B-009-001-MY3]
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A novel horseradish peroxidase-encapsulated silica nanoparticle (SNP) was generated in this study under relatively mild conditions. The generated enzyme-encapsulated SNP were relatively uniform in size (average 70 +/- 14.3 nm), monodispersed, and spherical, as characterized by transmission electron microscopy and scanning electron microscopy. The horseradish peroxidase encapsulated in silica nanoparticle exhibits biological properties, such as a pH-dependent activity profile and k(m) value, similar to that of free enzymes. Furthermore, enzyme-encapsulated SNP exhibited good operational stability for the repetitive usage with a relative standard deviation of 5.1 % ( n = 10) and a high stability for long term storage (>60 days) at 4 degrees C. The feasibility of using enzyme-encapsulated SNP in prodrug cancer therapy was also demonstrated by its capability to convert the prodrug indole-3-acetic acid into cytotoxic peroxyl radicals and trigger the death of tumor cells. These results indicate that the developed enzyme-encapsulated SNP has potential in the applications of prodrug cancer therapy.
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