Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 12, Issue 8, Pages 2729-2733Publisher
SPRINGER
DOI: 10.1007/s11051-010-0046-8
Keywords
Ratiometric fluorescence; Nanoparticles; Europium chelate; Temperature sensing
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Funding
- Alexander-von-Humboldt Foundation (Bonn)
- National Natural Science Foundation of China [10774012, 10874014]
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A ratiometric type of fluorescent nanoparticle was prepared via an encapsulation-reprecipitation method. By introducing an alkoxysilanized dye as a reference, the nanoparticles (NPs) give both a green and a red fluorescence under one single-wavelength excitation. The resulted ratiometric fluorescence is found to be highly temperature-dependent in the physiological range (25-45 A degrees C), with an intensity temperature sensitivity of -4.0%/A degrees C. Given the small size (20-30 nm in diameter) and biocompatible nature (silica out layer), such kind of NPs were very promising as temperature nanosensors for cellular sensing and imaging.
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