Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 11, Issue 2, Pages 279-288Publisher
SPRINGER
DOI: 10.1007/s11051-008-9378-z
Keywords
Gold nanoparticles; Extracellular biosynthesis; Monodispersity; Control of particle size; Self-assembled monolayer; Nanobiotechnology
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Funding
- National Natural Science Foundation of China [20433040, 20423002]
- State Key Laboratory for Physical Chemistry of the Solid Surface, Xiamen University of China [200408]
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Monodispersed gold nanoparticles capped with a self-assembled monolayer of dodecanethiol were biosynthesized extracellularly by an efficient, simple, and environmental friendly procedure, which involved the use of Bacillus megatherium D01 as the reducing agent and the use of dodecanethiol as the capping ligand at 26 A degrees C. The kinetics of gold nanoparticle formation was followed by transmission electron microscope (TEM) and UV-vis spectroscopy. It was shown that reaction time was an important parameter in controlling the morphology of gold nanoparticles. The effect of thiol on the shape, size, and dispersity of gold nanoparticles was also studied. The results showed that the presence of thiol during the biosynthesis could induce the formation of small size gold nanoparticles (< 2.5 nm), hold the shape of spherical nanoparticles, and promote the monodispersity of nanoparticles. Through the modulation of reaction time and the use of thiol, monodispersed spherical gold nanoparticles capped with thiol of 1.9 +/- A 0.8 nm size were formed by using Bacillus megatherium D01.
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