Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 11, Issue 4, Pages 775-783Publisher
SPRINGER
DOI: 10.1007/s11051-008-9424-x
Keywords
Aptamer; Gold nanoparticles; Breast cancer cells; Imaging; Proliferation; Nanomedicine
Categories
Funding
- National Science Council [NSC 95-2113-M-002-026-MY3, NSC 96-2627-M-002 -013, NSC 96-2627-M-002-014]
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In this study, we demonstrated the potential use of nucleic acid ligand (aptamers) conjugated gold nanoparticles (AuNPs) for cancer cell detection. Through specific binding of the aptamers toward platelet-derived growth factor (PDGF), MDA-MB-231 and Hs578T cells (cancer cells) that over-express PDGF, interact with Apt-AuNPs to a greater extent than do H184B5F5/M10 cells (normal cells). These results were confirmed through inductively coupled plasma mass spectrometry measurements of the gold ion concentrations within these cells. Aggregation of the Apt-AuNPs in the cytoplasm of the cancer cells led to the generation of an intense scattered light upon photo-illumination; this phenomenon allows the differentiation of cancer cells from normal cells using a dark field optical microscope. The presence of Apt-AuNPs suppressed the proliferation of MDA-MB-231 cancer cells, but not H184B5F5/M10 cells.
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