Journal
PHARMACOGNOSY MAGAZINE
Volume 11, Issue 44, Pages S585-S591Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/0973-1296.172966
Keywords
Anti-inflammatory effect; herbal medicine; high-performance liquid chromatography; RAW 264.7; Xanthii fructus
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Funding
- Korea Institute of Oriental Medicine [K15251]
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Objective: Xanthii fructus (Compositae) is a traditional herbal medicine used for treating headache, toothache, pruritus, empyema, and rhinitis. In this study of the quality control of XI fructos, we performed simultaneous analysis of nine marker compounds: Protocatechuic acid (1), chlorogenic acid (2), caffeic acid (3), 4, 5-dicaffeoylquinic acid (4), ferulic acid (5). 3,5-dicaffeoylquinic acid (6), 1,3-dicaffeoylquinic acid (7), 1,4-dicaffeoylquinic acid (8), and 4,5-dicaffeoylquinic acid (9). Materials and Methods: Nine components were separated using reversed-phase SunFire (TM) C-18 analytical column and analyzed using high-performance liquid chromatography. We examined the biological effects of the nine marker compounds by determining their anti-inflammatory activities in the murine macrophage cell line RAW 264.7 Results: Among the nine marker compounds, eight significantly inhibited lipopolysaccharide (LPS)-stimulated tumor necrosis factor-alpha (TNF-alpha) production. 1, 3, 5 had significant inhibitory effects on LPS-induced prostaglandin E-2 (PGE(2)) production in RAW 264.7 cells. None of the tested marker compounds had a significant effect on interleukin-6 production in LPS-treated RAW 264.7 cells. Our data demonstrated that each marker compound from X. fructus exerts anti-inflammatory activity by targeting different inflammation-related pathways such as the INF-alpha or PGE(2) pathway. Conclusion: Further experiments using in vitro and in vivo models are needed to identify the mechanisms responsible for the anti-inflammatory properties of each marker compound.
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