Journal
JOURNAL OF NANOMATERIALS
Volume 2013, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2013/317437
Keywords
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Funding
- AQSIQ Grant [2011IK249]
- national science foundation of Zhejiang province [Y3090211]
- program for science and technology from Zhejiang province [2011C22065]
- program for science and technology from Zhejiang entry-exit inspection and quarantine bureau [ZK200957, ZK200958, ZK201310]
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A sensitive, specific and rapid method for the detection of three different kinds of plant viruses including tomato ringspot virus (ToRSV), bean pod mottle virus (BPMV) and arabis mosaic virus (ArMV) was demonstrated using novel upconversion nanoparticles (UCNPs) as a fluorescence marker coupled with immunomagnetic separation. Magnetic nanoparticles (MNPs, similar to 100 nm) were coated with different antibodies were employed to capture and enrich the target viruses. Then antibody-conjugated UCNPs as signal probes were added to form sandwich complexes. This was followed by a fluorescence measurement using a 980 nm laser. This method not only avoids the difficulty in simultaneous detection of multiple independent organic fluorphores that require distinct excitation wavelengths, and auto-fluorescence of biological samples due to the higher-energy excitation of QDs but also amplifies detection signal by UCNPs-tags together with easy separation of samples by magnetic forces, demonstrating the potential to be used for detecting virus in the field of environmental safety and other fields.
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