Journal
PHARMACOGNOSY MAGAZINE
Volume 11, Issue 41, Pages 176-179Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/0973-1296.149735
Keywords
Amino acids; 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; Matricaria chamomilla; Matricaria recutita; reversed-phase high performance liquid chromatography
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Funding
- National Natural Science Foundation of China [81260144]
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Objective: To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. Materials and Methods: Derivatization with reconstituted AQC was used to prepare the samples and standards for injection into the chromatography column. The peaks were analyzed by fluorescence detection ( excitation, 250 nm; emission, 395 nm. Results: Alanine, proline, and leucine were the most abundant amino acids, whereas tyrosine and methionine were the least abundant. The linearity of the method was found to be good with amino acid concentrations of 0.012-0.36 M. The precision was 0.05-1.36%; average recovery, 91.12-129.41%; and limit of detection, 0.006-0.058 M. Conclusion: The method is reliable for determining the free amino acid content of different types of chamomile flowers.
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