Journal
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
Volume 29, Issue 6-8, Pages 169-172Publisher
SPRINGER
DOI: 10.1007/s10974-009-9170-4
Keywords
Troponin I; Phosphorylation; Cardiac; Hypertrophic cardiomyopathy; Heart failure; Phos-tag (TM); Pro-Q (R); Diamond; Phosphoprotein
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Funding
- National Heart and Lung Institute, Imperial College London
- British Heart Foundation [PG/08/077/25587, RG/02010, PG/06/027/20425]
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Cardiac troponin I phosphorylation has been shown to be reduced in human heart failure and hypertrophic cardiomyopathy using the increasingly popular Pro-Q(A (R)) Diamond phosphoprotein gel stain. In this brief report I discuss the use of Western immunoblotting, non-equilibrium isoelectric focussing and Pro-Q(A (R)) Diamond and introduce phosphate affinity SDS-PAGE using Phos-tag (TM)-acrylamide for the investigation of troponin I phosphorylation.
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