Journal
JOURNAL OF MOLECULAR STRUCTURE
Volume 1023, Issue -, Pages 197-203Publisher
ELSEVIER
DOI: 10.1016/j.molstruc.2012.04.031
Keywords
Microarray chip; Hollow gold nanospheres; Surface-enhanced Raman scattering; Multiplex assay; Immunoassay; ELISA
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Funding
- National Research Foundation of Korea [R11-2008-0061852, K20904000004-09A0500-00410]
- Agency for Defense Development through Chemical and Biological Defense Research Center
- Higher Education Commission (HEC) Program of the government of Pakistan
- Ministry of Knowledge Economy (MKE)
- Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology
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We report a highly sensitive surface-enhanced Raman scattering (SERS)-based immunoassay platform for the multiplex detection of biomarkers. For this purpose, a gold-patterned microarray chip has been fabricated and used as a SERS detection template. Here, a typical sandwich immunocomplex protocol was adopted. Monoclonal antibodies were immobilized on gold patterned substrates, and then antigen solutions and polyclonal antibody-conjugated hollow gold nanospheres (HGNs) were sequentially added for the formation of sandwich immunocomplexes. Antigen biomarkers can be quantitatively assayed by monitoring the intensity change of a characteristic SERS peak of a reporter molecule adsorbed on the surfaces of HGNs. Under optimized assay conditions, the limits of detections (LODs) were determined to be 10 fg/mL for human IgG and 10-100 fg/mL for rabbit IgG. In addition, the SERS-based immunoassay technique can be applied in a wider dynamic concentration range with a good sensitivity compared to ELISA. The proposed method fulfills the current needs of high sensitivity and selectivity which are essential for the clinical diagnosis of a disease. (c) 2012 Elsevier B.V. All rights reserved.
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