Journal
JOURNAL OF MOLECULAR STRUCTURE
Volume 998, Issue 1-3, Pages 144-150Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molstruc.2011.05.023
Keywords
Fluorescence quenching; Ligand binding; Fluorescence spectroscopy; Inner-filter effect; Forster Resonance Energy Transfer; Hill equation
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In recent years fluorescence quenching has become a popular tool to investigate various aspects of ligand binding. Unfortunately, various pitfalls are often overlooked in a large number of papers, published in many different journals. In this criticism we discuss a number of possible mistakes and show how they may affect the data and their analysis. Moreover, we point to problems in the understanding of the fundamentals of fluorescence quenching, and show direct contradictions within many of these papers. This review hopefully contributes to a re-appraisal of the published literature and to a more appropriate use of fluorescence quenching to study ligand binding. (C) 2011 Elsevier B.V. All rights reserved.
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