4.2 Article

Label-free characterization of carbonic anhydrase-novel inhibitor interactions using surface plasmon resonance, isothermal titration calorimetry and fluorescence-based thermal shift assays

Journal

JOURNAL OF MOLECULAR RECOGNITION
Volume 27, Issue 1, Pages 46-56

Publisher

WILEY-BLACKWELL
DOI: 10.1002/jmr.2330

Keywords

surface plasmon resonance; isothermal titration calorimetry; fluorescence-based thermal shift assay; carbonic anhydrase; kinetics; thermodynamics; binding; diarylpyrazole sulfonamide

Funding

  1. Ligue Nationale contre le cancer, Comite du Pas-de Calais
  2. PRIM, Pole de Recherche Interdisciplinaire du Medicament (Region Nord-Pas-de-Calais, Ministere delegue a la Recherche et aux Nouvelles Technologies, European Union (Fonds europeen de developpement regional (FEDER)
  3. GEFLUC, Groupements des Entreprises Francaises dans la Lutte contre le Cancer, Comite du Nord Pas-de Calais

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This work describes the development of biophysical unbiased methods to study the interactions between new designed compounds and carbonic anhydrase II (CAII) enzyme. These methods have to permit both a screening of a series of sulfonamide derivatives and the identification of a lead compound after a thorough study of the most promising molecules. Interactions data were collected using surface plasmon resonance (SPR) and thermal shift assay (TSA). In the first step, experiments were performed with bovine CAII isoform and were extended to human CAII. Isothermal titration calorimetry (ITC) experiments were also conducted to obtain thermodynamics parameters necessary for the processing of the TSA data. Results obtained with this reference methodology demonstrate the effectiveness of SPR and TSA. K-D values obtained from SPR data were in perfect accordance with ITC. For TSA, despite the fact that the absolute values of K-D were quite different, the same affinity scale was obtained for all compounds. The binding affinities of the analytes studied vary by more than 50 orders of magnitude; for example, the K-D value determined by SPR were 6 +/- 4 and 299 +/- 25nM for compounds 1 and 3, respectively. This paper discusses some of the theoretical and experimental aspects of the affinity-based methods and evaluates the protein consumption to develop methods for the screening of further new compounds. The double interest of SPR, that is, for screening and for the quick thorough study of the interactions parameters (k(a), k(d), and K-D), leads us to choose this methodology for the study of new potential inhibitors. Copyright (c) 2013 John Wiley & Sons, Ltd.

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