Journal
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
Volume 22, Issue 2, Pages 83-93Publisher
KARGER
DOI: 10.1159/000338530
Keywords
Bacillus subtilis XF-1; Fengycins; Antifungal fraction; LC/ESI-MS/MS
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Funding
- Ministry of Agriculture of China Special Fund for Agro-Scientific Research in the Public Interest [2010029030]
- Natural Science Foundation of Yunnan Province [2008CC024]
- National Natural Science Foundation of China [20972166, 31070288, 31161140345]
- Ministry of Education of China [B08044, MUC 985-6, MUC 98506-01000101]
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Bacillus subtilis XF-1 (CGMCC No. 2357), a patent strain with good effects for controlling the clubroot of crucifer and many pathogenic fungi, was predicted to produce cyclic lipopeptide (CLP) antibiotics based on its genomic analysis. In this study, the CLPs were purified and determined with the following protocol: the supernatant of XF-1 cultivating mixture was firstly precipitated, then the precipitants were extracted with methanol and further separated by Sephadex LH-20 chromatography to test its antifungal activities. Fungi-inhibiting fractions were further characterized with LC/ESI-MS and LC/ESI-MS/MS. The results show that four molecular ion peaks [M+H](+) (m/z 1,464, 1,478, 1,492 and 1,506) from fungi suppression fraction were identified as fengycin A with fatty acid of C-16-C-19, fengycin B (C-14-C-17), fengycin C (C-15-C-18), fengycin D (C-15-C-18) and fengycin S (C-15-C-18). Fengycin C (C-15 and C-18), fengycin D (C-15, C-16 and C-18) and fengycin S (C-15, C-16 and C-18) were reported for the first time. The diversity of the fengycins that exist in this strain will help the elucidation of their biocontrol mechanisms. Copyright (C) 2012 S. Karger AG, Basel
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