Journal
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
Volume 18, Issue 2, Pages 92-101Publisher
KARGER
DOI: 10.1159/000287988
Keywords
'Aromatoleum aromaticum' strain EbN1; Denitrification; Anaerobic aromatic compound degradation; Solvent stress; Bacterial phospholipids; Liquid chromatography-mass spectrometry; Molecular adaptation
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Funding
- Max-Planck-Gesellschaft
- Deutsche Forschungsgemeinschaft (DFG)
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The denitrifying betaproteobacterium strain EbN1 degrades toluene and ethylbenzene under anoxic conditions. Alkylbenzenes are unusual substrates, since their extraordinary chemical stability necessitates complex reactions and their toxic properties as solvents challenge cellular viability. To study the solvent impact on membrane lipid composition, strain EbN1 was grown at low, standard and semi-inhibitory concentrations of toluene (70, 240, and 740 mu M) and ethylbenzene (80, 210, and 315 mu M). At semi-inhibitory concentrations, phosphatidylglycerol increased at the expense of phosphatidylethanolamine. Moreover, phosphatidylcholine proportions increased and tentatively identified N-hexanoyl-phosphatidylethanolamine was detected. All observed changes in membrane lipid composition are interpreted as the organism's response to prevent the maceration of the cell membrane. Copyright (C) 2010 S. Karger AG, Basel
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