Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 117, Issue -, Pages 39-46Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2014.10.006
Keywords
Phenazine-1-carboxylic acid; Xanthomonas oryzae pv. oryzae; Biological effects
Categories
Funding
- National Key Basic Research Program (973 Program) [2009CB118906]
- National Natural Science Foundation of China [31272065]
- Jiangsu Province Postgraduate Innovative Project [CXZZ12_0292]
- Opening Project of State Key Laboratory of Microbial Metabolism [MMCKF13-02]
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Xanthomonas oryzae pv. oryzae (Xoo) is the casual agent of bacterial blight, which is one of the most serious diseases of rice. The antibiotic phenazine-1-carboxylic acid (PCA), which is primarily produced by Pseudomonas spp., was found and previously reported very effective against Xoo. However, the biological effects of PCA on Xoo remain unclear. In this study, we found that PCA increased the accumulation of reactive oxygen species (ROS) and reduced the activities of catalase (CAT) and superoxide dismutase (SOD) in Xoo. Xoo was more sensitive to H2O2 than Xanthomonas oryzae pv. oryzicola (Xoc), and had a much lower expression of CAT genes. In addition, proteomic analysis indicated that PCA inhibited carbohydrate metabolism and nutrient uptake in Xoo, and analysis of carbon source utilization further confirmed that carbohydrate metabolism in Xoo was repressed by PCA. In conclusion, PCA acted as a redox-cycling agent that disturbed the redox balance in Xoo and reduced CAT and SOD activities, resulting in higher accumulation of ROS, altered carbohydrate metabolism, and lower energy production and nutrient uptake. Moreover, a deficient antioxidant system in Xoo made it very sensitive to PCA. (C) 2014 Elsevier Inc. All rights reserved.
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