Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 109, Issue -, Pages 154-160Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2014.08.011
Keywords
Mesoporous silicates; Metal cyclam; His-tagged alanine racemase
Funding
- Science Foundation Ireland [RFP06/CHP001]
- Irish Research Council
- HEA PRTLI4 programme, INSPIRE
Ask authors/readers for more resources
Nickel and cobalt cyclam modified mesocellular foam (MCF) materials were prepared and characterised. The metal cyclam modified materials displayed reduced surface areas and pore diameters in comparison to MCF. The modified materials were used to specifically anchor a histidine tagged form of the enzyme, alanine racemase (HT-AlaR). Non-specific adsorption was predominantly hydrophobic//hydrophilic in nature and could be significantly reduced in the presence of 2% polyethylene glycol. The activity of HT-AlaR immobilised on Ni and Co-MCF was essentially the same as that of the free enzyme, demonstrating that enzymes can be specifically immobilised within the pores of mesoporous materials in a stable and catalytically active manner. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available