Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 76, Issue -, Pages 89-93Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2011.11.013
Keywords
Efflux pump; AcrAB-ToIC; Alcohol tolerance
Funding
- A*STAR in Singapore [ICES/07-173B01]
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We investigated the alcohol tolerance of Escherichia coli acrR and marR regulatory mutants. The local repressor gene acrR and the global repressor gene marR were deleted either separately or together from the genomic DNA of E. coli for overexpression of AcrAB-ToIC. Mutants with Delta acrR background indicated no growth difference in the exponential growth phase and modestly better growth in the stationary growth phase than the parental strain under various concentrations of alcohol. However, single marR mutant did not show any better growth than the parental strain in the presence of butanol and isobutanol although the efflux pump AcrAB-ToIC was upregulated. (C) 2011 Elsevier B.V. All rights reserved.
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